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1: Biofizika. 2002 Mar-Apr;47(2):295-9. Related Articles, Links: [The role of reactive oxygen species in copper-induced permeability of plasma membranes in Escherichia coli]

[Article in Russian]

Lebedev VS, Veselovskii AV, Deinega EIu, Fedorov IuI.

Institute of Energy Problems of Chemical Physics, Russian Academy of Sciences, Leninskii pr. 38/2, Moscow, 117829 Russia.

The role of active oxygen species in the induction of nonselective cationic permeability of the plasma membrane of bacteria Escherichia coli B by the action of Cu2+ ions was studied. It was found that the increase in the amount of active oxygen species in the suspension after treating cells with copper occurred synchronously with the leakage of K+ cations from them. Evidence is presented that active oxygen species formed during the interaction of copper ions with bacteria under aerobic conditions are not involved in the induction of channel conductivity in the membrane. Moreover, the ability of oxygen to protect the membrane from the toxic action of copper was shown, and the activation of membrane damage by external reductants was confirmed. These data suggest that the barrier properties of the membrane are disturbed during the interaction of Cu+ ions with critical targets on the surface, the concentration of Cu+ being determined by all redox processes in the near-membrane space.

PMID: 11969166 [PubMed - indexed for MEDLINE]