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Lebedev V.S., Veselovsky A.V., Fedorov Yu.I. We investigated the ability of hydrophilic impermeant (EDTA) and hydrophobic
permeant (8-hydroxyquinoline) copper complexing agents to eliminate the
Cu2+-induced functional disturbances in membrane of Escherichia coli cells. Both
agents efficiently reversed Cu2+-induced K+ leakage and membrane depolarization.
The respiration rate was, however, restored only in the presence of the permeant
complexing agent. The results are consistent with the view that Cu2+-induced
membrane permeability is dynamic, is due to the interaction of copper ions with
the targets on the outer side of membrane and disappears in the absence of
exogenous energy sources when the metal ions are removed from the medium.
Transport of Cu2+ into the cytoplasm is through pores in membrane by an electric
field gradient. Copper thus accumulated is inaccessible for the impermeant
complexing agent and is responsible for the inhibition of respiration when Cu2+
ions are removed from the medium. |
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